Ku protein.

Figure S3: Characterization of a Ku trans-complemented Ku80 +/− HCT 116 clone. Wild-type (WT) and Ku80 +/− human colon carcinoma HCT 116 cells as well as derivatives containing either a neomycin (Neo) or a hygromycin-Ku80 expression pcDNA3.1 plasmid (shortened as 'pCtl' and 'pKu80', respectively) were subjected to Western-blot assessment of Ku80 protein contents.15-10-2023. PhD fellowship in Experimental Quantum Optics, Niels Bohr Institute. Faculty of Science. Niels Bohr institutet. 15-10-2023. 160061 Associate Professor of cognitive neurorehabilitation (Part time, Fixed-Term, 5 Years) Faculty of Health and Medical Sciences. Department of Neuroscience. 15-10-2023.LAWRENCE — Research from the University of Kansas just published in Proceedings of the National Academy of Sciences could hasten development of a new class of vaccines aimed at SARS-CoV-2, the virus that causes COVID-19.. Anthony Fehr, associate professor of molecular biosciences, led research into a protein dubbed "Mac1," which has intrigued molecular bioscientists as an antiviral ...Besides these inhibiting molecules, HDR can also be promoted using small interfering RNA (siRNA) to inhibit the expression of Ku protein, which is the pioneer protein in the NHEJ pathway. Li et al. assessed this method on pig fetal fibroblasts (Li et al., 2018). The result showed that by inhibiting Ku70 or Ku80 expression, HR can be promoted by ...Yeast KU protein 1 Comparative Info. Sequence Details Sequence The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains. ...

Mar 27, 2021 · In yeast, KU protein slides onto the DNA end and translocates inward. Ku interacts with the DNA ligase IV complex. The catalytic subunit of DNA ligase IV, along with its co-factors Dnl4 and Lif1, performs the DNA repair. Recent studies suggest that in yeast, the Nej1, which is homologous to mammalian XLF, may promote re-adenylation of DNA ...The Ku heterodimer, consisting of two subunits, Ku70 and Ku80, is a member of a family of DNA repair proteins that fixes damaged DNA in order to preserve the integrity of the genome. When Ku encounters damaged DNA, it initiates a repair process, called non-homologous end joining (NHEJ), which stitches double-stranded broken ends back together ...Here, small angle X-ray scattering (SAXS) and mutational analyses show APLF is largely an intrinsically disordered protein that binds Ku, Ku/DNA-PKcsmore » X4L4 assembles with Ku heterodimers linked to DNA-PKcs via flexible Ku80 C-terminal regions (Ku80CTR) in a complex stabilized through APLF interactions with Ku, DNA-PK, and X4L4.

We explore unique structural and functional aspects, detail Ku expression, conservation, and essentiality in different species, discuss the evidence for its involvement in a diverse range of cellular functions, highlight Ku protein interactions and recent work concerning Ku-binding motifs, and finally, we summarize the clinical Ku-related ...

Protein-peptide interactions play a fundamental role in many cellular processes, but remain underexplored experimentally and difficult to model computationally. Here, we present PepNN-Struct and ...Ku (red circle and red rectangle) is the most abundant DNA end binding protein in eukaryotic cells and can slide onto DNA ends that have diverse configurations . Once Ku is bound to the DNA end, it can improve the binding equilibrium of the nuclease, polymerases and ligase of NHEJ.In this study, we have studied the interaction of Ku with nucleic acids quantitatively by following the change in fluorescence anisotropy of the DNA duplex associated with protein binding. The binding of Ku to nucleic acids occurs at DNA ends and is independent of their structure and sequence (. 10.The DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase-related kinase family, phosphorylates serine and threonine residues of substrate proteins in the presence of the Ku complex and double-stranded DNA. Although it has been established that DNA-PKcs is involved in non-homologous end-joining, a DNA double-strand break repair pathway, the ...Jun 15, 2017 · Subsequent work established that effective DNA-PKcs recruitment and activation by DNA require Ku, a heterodimer comprising two subunits of ∼70 and 80 kDa (Dvir et al., 1992, ... One such protein is the p53 co-factor hnRNP K, which is phosphorylated by ATM in response to DSBs and protected from MDM2-mediated degradation (Moumen …

Coilin is a nuclear protein that plays a role in Cajal body formation. The function of nucleoplasmic coilin is unknown. Here we report that coilin interacts with Ku70 and Ku80, which are major players in the DNA repair process. Ku proteins compete with SMN and SmB′ proteins for coilin interaction sites.

The protein expression of Ku 70 and Ku 80 was closely related and decreased consistently with increasing grades and stages, paralleling the expression of the mRNA. Among hazardous environmental bladder cancer risk factors, heavy consumption of coffee was associated with a twofold decreased Ku 70 and Ku 80 mRNA expression, whereas tobacco smoke ...

We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo. In vitro, Ku70-/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku …Jul 27, 2021 · They demonstrated that Ku is an essential component of DNA-PK, mediating the binding of DNA-PKcs to the dsDNA end . The Ku protein was initially identified by Mimori et al. as the antigen of the autoantibody in a patient of an autoimmune disease, polymyositis–scleroderma overlap . Ku is an abundant protein that has an extremely high affinity for dsDNA ends that forms a ring-shaped protein that slides onto the ends of the broken DNA molecule in a sequence independent manner [13–16].Sep 14, 2020 · The mixture was excited at 435 nm, and emission was recorded between 440 and 650 nm. One microgram of ECFP-Ku70/EYFP-Ku80 (or a Ku mutant protein) was incubated for 60 minutes in 80 µL FRET buffer (25 mM Tris-HCl pH8.0, 120 mM KCl, 10 mM MgCl 2, and 2 mM DTT). DNA oligonucleotide substrates were prepared in FRET buffer containing 2 μg ... Oxidative stress induces DNA damage which can be repaired by DNA repair proteins, such as Ku70/80. Excess reactive oxygen species (ROS) stimulate the activation of caspase-3, which degrades Ku 70/80. Cells with decreased Ku protein levels undergo apoptosis. Astaxanthin exerts antioxidant activity by inducing the expression of catalase, an antioxidant enzyme, in gastric epithelial cells ...The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3′→5′ helicase and 3′→5′ exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functionally.Amazon.in - Buy KU-Protein Structure of Mycobacterium Tuberculosis along with the Interaction Involving KU-Protein and DNA Polymerase book online at best ...

We have characterized the biochemical nature of the Ku protein, the antigen recognized by autoantibodies from certain patients with scleroderma-polymyositis overlap syndrome. From extracts of HeLa cells labeled with [32P]orthophosphate, anti-Ku antibodies precipitated a high molecular weight nucleic acid identified as DNA because of sensitivity ...The Ku autoantigen is a DNA binding factor consisting of 70 and approximately 80 kDa proteins (p70 and p80, respectively) which form a heterodimer. The p70/p80 dimer appears to be crucial for the function of a 350 kDa DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription fa …Ku is an abundant nuclear protein that consists of two polypeptides of molecular masses 80-86 and 70-72 kDa. It was first identified in the serum of a patient with scleroderma polymyositis overlap syndrome (), and antibodies against this protein have been detected in the sera of patients with various autoimmune diseases ().It can bind to the DNA ends (2, 3) as well as the internal DNA ...The inability to rescue activity with purified Ku suggests either that the role of Ku is indirect or that the endogenous Ku differs in some way from the exogenous protein. Interestingly, a different CHO cell mutant that lacks the DNA-dependent protein kinase catalytic subunit, a protein in the same repair pathway as Ku, also exhibits a decrease ...Unexpectedly, we found that abolishing Ku protein gene function perturbs mating type determination and generates selfing clones in Tetrahymena. Detailed analysis uncovers unusual DNA rearrangement steps in Tetrahymena mating type determination, sheds light on the molecular nature of self-nonself-discrimination, and reveals an unexpected phase ...Abstract. Ku protein is a heterodimer (Ku70 and Ku86) known to play an important role in V(D)J recombination, apoptosis, telomere fusion, and double-strand break repair. Its role in double-strand breaks is relevant to cancer therapy because lack of Ku86 causes one of the most radiation-responsive phenotypes (hamster cells, XRS5). Although it is known that the heterodimer is necessary for the ...

KU protein can exert many cellular functions, including modification of RNA, DNA replication, innate immunity、DNA damage repair and telomere maintenance. Telomeres are specialized structures at the ends of eukaryotic chromosomes that are important for maintaining genome stability and integrity [21]. Shortening of telomeres is associated with ...The Ku protein binds to DNA ends and other types of discontinuity in double-stranded DNA. It is a tightly associated heterodimer of ∼70 kDa and ∼80 kDa subunits that together with the ∼470 kDa catalytic subunit, DNA-PKcs, form the DNA-dependent protein kinase. This enzyme is involved in repairing DNA double-strand breaks (DSBs) caused ...

Correspondingly, Ku forms the DNA-binding portion of the trimeric DNA-dependent protein kinase (DNA-PK) holoenzyme (Gottlieb and Jackson, 1993), in which the C-terminus of Ku80 facilitates the recruitment and assembly of the kinase DNA-PKcs on DNA ends (Falck et al., 2005) (discussed further under ' End Processing '). Thus, while the full ...Ku is the regulatory subunit of the DNA-dependent protein kinase (DNA-PK). This enzyme plays a role in DNA repair, recombination, and transcription.30°C in the presence of 20 nM Ku protein, 18.5 nM DNA-PKcs, 70 µM peptide and 40 µM[γ-32P]ATP (12.5 Ci/mmol) in a total volume of 20 µl. Reactions were stopped by addition of 10 µlofThe inability to rescue activity with purified Ku suggests either that the role of Ku is indirect or that the endogenous Ku differs in some way from the exogenous protein. Interestingly, a different CHO cell mutant that lacks the DNA-dependent protein kinase catalytic subunit, a protein in the same repair pathway as Ku, also exhibits a decrease ...Recombinant untagged Ku protein (50 nM) was then incubated with the beads for 1 h at 4 °C, the unbound fraction removed and washes performed as above. Bound proteins were eluted from the beads ...The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72 , 131-142 (1993). Article CAS PubMed Google ScholarKu80, a subunit of the Ku protein complex, is an initiator of the non-homologous, end-joining (NHEJ), double-strand breaks (DSBs) repair pathway. The nuclear Ku80 was cleaved in a calcium concentration-dependent manner by m-calpain but not by m-calpain. The cleavage of nuclear Ku80 at its α/β domain was validated by Western blotting analysis ...The Ku protein, a DNA-binding complex that is composed of two subunits of 70 kDa and of 86 kDa, has been suggested to play a role in gene transcription. The dependence of the in vitro DNA-binding ...

In addition, mycobacteria contain a homo-dimeric Ku protein, homologous to the eukaryotic Ku70/Ku80 dimer, that plays roles in double-stranded break repair via non-homologous end-joining. Ku has ...

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Phosphorylated H2AX recruits and phosphorylates Mediator of Damage Checkpoint protein 1 ... dependent protein kinas e phosphorylatio n sites in Ku 70/80. heterodimer, ” Biochemistry, vol. 38, no ...Sep 27, 2017 · The prototypical NHEJ protein is Ku, which is a heterodimer of Ku70 and Ku80. Upon the occurrence of DSBs, the Ku protein complex binds to the ends of DSBs. The DNA ligase is then recruited to join the DNA ends together. Since the Ku factor allows random joining of any two ends of DSBs, mutation occurs upon the end-joining of ends …Jul 11, 2017 · DNA-dependent protein kinase (DNA-PK) is a large protein complex central to the nonhomologous end joining (NHEJ) DNA-repair pathway. It comprises the DNA-PK catalytic subunit (DNA-PKcs) and the heterodimer of DNA-binding proteins Ku70 and Ku80. Here, we report the cryo-electron microscopy (cryo-EM) … The human Ku protein used in this study was prepared from baculovirus as described previously (43), with the cDNA for the p70 subunit encoding a histidine tag used to purify the Ku heterodimer by nickel affinity chromatography (Fig. 2 A).This form of Ku recognizes NRE1 indistinguishably from human Ku purified from Jurkat T cells (38).In the first experiment (Fig. 2 B), we compared Ku binding ...Through protein-protein contacts eventually reinforced by protein-DNA interactions, the Ku-DNA hub attracts a series of specialized proteins with scaffolding and/or enzymatic properties.In reactions in which the addition of one protein preceded the other, the DNA substrate was incubated with the first protein for 10 min, followed by the second protein and a 10-min incubation. The DNA-Ku-RPA ternary complex is indicated by the arrow.Structural proteins are used to build structural components of the body, such as bones and cartilage. The most common example of a structural protein is collagen which is found in the bones, cells and skin.(C) Electrophoretic mobility shift assay of radiolabeled ds DNA probe incubated with purified human recombinant Ku protein in the presence of various competitor ssO bearing a biotin moiety at the 3′-, 5′- or 3′- and 5′- end, blocked or not by streptavidin, as indicated. ds probe and ssO amount in reactions were 0.2 and 10 pmol ...The Ku protein exists as a homodimer and preferentially binds to dsDNA ends (Weller et al., 2002). LigD is an adenosine triphosphate (ATP)-dependent DNA ligase that contains polymerase and nuclease domains, which facilitates the joining of long linear DNA molecules with different incompatible ends (Della et al., 2004).For many of these companies, it represents a significant philosophical shift in policy. The “Unite the Right” rally in Charlottesville, Virginia, ostensibly a protest against the removal of a Confederate monument, drew hate groups that incl...Although 15/174 cannot be regarded as monospecific for anti-dsDNA, we found the raw material for 15/174 to be rather specific for anti-chromatin reactivities and antibodies against Ku, a DNA binding protein.12–14 It is …Aravind, L. & Koonin, E.V. Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system.

Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified by researchers as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis, and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA ...Oxidative stress induces DNA damage which can be repaired by DNA repair proteins, such as Ku70/80. Excess reactive oxygen species (ROS) stimulate the activation of caspase-3, which degrades Ku 70/80. Cells with decreased Ku protein levels undergo apoptosis. Astaxanthin exerts antioxidant activity by inducing the expression of catalase, an antioxidant enzyme, in gastric epithelial cells ...The effect of MCL1 on DSBs goes beyond its effects on DDR protein foci accumulation. MCL1 binds and inhibits Ku, an essential NHEJ protein that stabilizes the DNA ends to promote ligation 54 (Fig ...Instagram:https://instagram. research library proquestbiol 401dsw orland park hoursr h cooper The expressions of proteins of Ku 70 in B-cell lines (T13-1 cleus or cytoplasm might be related with slower movement of from α-rays-irradiated cells and T8-4 from γ-rays- irradiated Ku 70 protein, although role of Ku protein is poorly understood. cells) were slightly lower than T14-2 from non-exposed cells, af- Similar finding to those in ...The Mechanism of V(D)J Recombination. JOANN SEKIGUCHI, ... MARJORIE OETTINGER, in Molecular Biology of B Cells, 2004. Ku70 and Ku80. Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986).Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was … whs loginvegasinsider ncaa basketball Aug 2, 2019 · This mechanism is enhanced via the Shielden complex, which starts from Ku protein complex attaching to the ends of DNA that have been exposed. The broken DNA is later stabilized by DNA-PKcs. End processing of DNA is performed after the recruitment of Artemis nuclease complex. DNA ligase IV then ligates the ends of DNA to repair the … theater lawrence kansas This supports the notion that Ku70 may stabilize Ku86 protein. 21, 22 Ku proteins have been reported to support cell survival but the mechanism(s) involved is not fully understood. 20, 23 To test ...Furthermore, the Ku protein plays essential roles in protecting DSB against resection and in promoting subtelomeric gene repression (Boulton and Jackson 1996, 1998). More recently, artificially tethering the ATM checkpoint kinase Tel1 or the DNA repair protein Mre11 to a defective silencer was shown to promote silencing at this locus.