Blue pippin.

Empress plum. Empress, also widely-known as Blue Imperatrice, is an unusually late-season dark blue / purple European plum. The plums have a sweet sugary flavor, but the golden yellow flesh is quite dry. The plums are "persistent", and if not picked will hang on the tree well into late fall. They can also be kept in a fridge for several weeks.Pippin Hill Farm and Vineyard is a wedding venue located in North Garden, VA. Nestled in the foothills of the Blue Ridge Mountains, this property is a romantic destination for an unforgettable wedding celebration. Every detail of this captivating property has been carefully considered by the venue's master planning team with wedding events in ...Technical note ALTERNATIVE SIZE SELECTION METHODS FOR SMRTBELL ® PREP KIT 3.0 Introduction In Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0, size-selection beads are used to remove short DNA fragments and enrich for the long fragments that are most valuable for …The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10ug (100% up from standard Blue Pippin cassettes).

The pooled libraries were then cleaned up with 0.45X AMPure PB beads (Pacific Biosciences) and subjected to size-selection on Blue Pippin (SAGE Science) in order to remove DNA fragments < 7 kb. The completed 20-22-plex pools were annealed to sequencing primer V4 and bound to sequencing polymerase 2.0, and were sequenced using one SMRTcell 8 M ...Abstract. The PacBio ® HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10–25 kb and accuracies greater than 99.5%. These accurate long ...

BluePippin Operations Manual 460013 Rev K 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anDouble digest restriction-site associated sequencing (ddRAD-seq) is a flexible and cost-effective strategy for providing in-depth insights into the genetic architecture of germplasm collections.

The Pippin facilitates library construction for the most popular NGS platforms. The platform features the ability to collect narrow and even fragment ...Blue Pippin Usage Form Date: Name: PI Name: Account #: Billing Contact: Billing Address: Cassettes Used (enter number): Any problems encountered during run? ITF Notes: Date Billed: Fill form: Try Risk Free. Form Popularity .50 years ago, on October 23, 1972, the Broadway cast of a new musical called Pippin had parts to perform and hearts to warm on their opening night at the Imperial Theatre. For the show's composer and lyricist, Stephen Schwartz, fresh off the success of Godspell Off-Broadway, the opening marked the premiere of his first Broadway musical. Pippin follows the journey of a young prince searching ...It's important to carefully inspect gel cassettes prior to use on the Pippin Prep or BluePippin. This instructional video from Sage Science shows how to quic...

BluePippin and Pippin HT. The Pippin systems from SageScience allow for precise automated size selection of nucleic acid samples, sequencing libraries, and even protein samples. Our Core runs both the BluePippin and the Pippin …

Page 5 Experimental Protocol STEP Preparing RiboMinus Magnetic Beads Notes 1 Resuspend the RiboMinus Magnetic Beads in its bottle by thoroughly vortexing. 2 For each sample, pipet 250 μL of the bead suspension into a sterile, RNase-free, 1.5 mL LoBind tube. 3 Place the tube/s with the bead suspension on a magnetic rack for 1 minute. Gently aspirate and discard the supernatant.

selection with either AMPure PB beads (red) or the BluePippin system (blue). Since the genomic DNA were partially degraded, SMRTbell Libraries were constructed without additional fragmentation. Long continuous read lengths are essential for applications such as de novo assembly and Isoform Sequencing (Iso-Seq).Happy Birthday Card with Sprinkles in Blue. A fun card to send to a friend or family member and wish them a special day, perfect for kids and adults!PippinHT Quick Guide HTC2010 Marker 20B. 1MB. PippinHT Quick Guide HTC1510 Marker 15C. 1MB. PippinHT Quick Guide HPE7510 Marker 75E 6-10Kb and 15-20kb High Pass. 948KB. PippinHT Quick Guide HPF7510 Marker 75F >30-40kb High Pass. 1MB. PippinHT Quick Guide HRT7510 9-30kb Range +T Marker 75E.Pippin definition, any of numerous roundish or oblate varieties of apple. See more.A disabled black veteran who taught himself to paint, Pippin overcame his handicap — as well as discrimination — to become an overnight sensation in the American art world in the 1940s. Many consider him to be the foremost self-trained American artist of the 20th century, a painter whose artwork is reminiscent of the work of Grandma Moses ...Check out the latest papers citing automated DNA size selection from Sage Science. Check out our new app note with @Diagenode! Best practices when using #Megaruptor3 w/ #PippinHT or #BluePippin. Includes our new Range+T size selection method & tips on getting tight HMW DNA in the 9-30kb range. #PacBio #HiFisequencing #nanopore #genomics https ...

Page 3 P/N100-377-100-05 1. For each sample and Control Mouse Liver Total RNA, combine the reagents below in separate PCR tubes. For polyA+ RNA, the minimum is 1 ng; total RNA requires 2 ng.The genomic DNAs were broken up by 26G Needle, and subsequently, the fragments above 10 kb were selected by Blue Pippin. After terminal repair and A-tailing, the ends of the fragments larger than 10 kb were ligated to generate the DNA library. The library sequencing was performed on a Pacbio sequel platform (Pacific Bioscience, California, USA ...The intestinal microbiota plays a crucial role in protecting the host from pathogenic microbes, modulating immunity and regulating metabolic processes. We studied the simplified human intestinal microbiota (SIHUMIx) consisting of eight bacterial species with a particular focus on the discovery of novel small proteins with less than 100 amino acids (= sProteins), some of which may contribute to ...SMRTbell library (10-20 kb in size) was prepared by the ligation of hairpin adaptors at both ends, and the resulting library was selected according to size using Blue Pippin with a 7-10 kb cutoff for Pacbio sequencing. Illumina Novaseq sequences were assembled using CLC Genomics Workbench 10 (CLC Bio, Aarhus, Denmark).2.0 BLUE-PIPPIN INSTALLATION REQUIREMENTS 2.1 Operating Conditions The BluePippin system is designed to operate under the following environmental conditions: Pollution Degree 2 Installation category 2 Max. Altitude 2000m Indoor use Ambient temperature 17-25oC Humidity 10-80%, non-condensing 2.2 Safety the gel. The analytical gel was run with a Pippin Pulse using the 10-48kb pre-set protocol and run for 15 hours. 0.75% SeqKem Gold agarose from Lonza was used. 4 kb 5 kb 8 kb 6 kb 10 kb None (Control DNA) 6 kb 7 kb 8 kb 10 kb High-Pass threshold setting 29 kb 36 kb Marker S1 At the end of a run, marker peaks will be detected in designated ...

selected protocol will be highlighted in blue. g. For the target duration, enter the desired run time (typically 14 to 16 hours). Note that the default value is 2 hours. Consider optimizing the run time based on the ambient lab temperature since the Pippin Pulse Electrophoresis System does not regulate the temperature in the gel chamber.

Size select the DNA with Blue Pippin, using High Pass Plus gel cassette and 0.75% Dye Free for 1–10 kb External Standards S1 according to manufacturer’s protocol. Further, 10 μg of DNA is required for each lane, and typically the amplified sample is enough for two lanes. Adjust the cutoff length depending on the fragment size distribution.The color blue symbolizes many things, including depth and stability. Often considered a male color, blue is also considered to be correlated with cold or cool, and calm, as it is the color of the sky and sea.The library was size selected (15-80 kb) using the Blue Pippin size selection instrument (Sage Science) and then sequenced on six SMRT cells (one cell at 2 pM and five cells at 6 pM) using 1 M ...Molecular characterization is a key step in the risk assessment of genetically modified organisms (GMOs) for regulatory approval. Herein, we describe a method for analyzing copy number, insertion loci, and flanking sequences through whole-genome sequencing (WGS) and bioinformatics. Comprehensive molecular characterization of G2-6 transgenic rice was performed using this pipeline. The results ...Laboratory for Biotechnology and Bioanalysis Purchase Request ...Blue pippin size selection elution volume 07-03-2015, 02:20 AM. Hello, Has anyone has tried eluting their TruSeq DNA libraries in a smaller volume than the 40uL stated in the protocol? We carry out size selection on our TruSeq nano libraries after the PCR enrichment step which works for us but gives very low concentrations. I was wondering if ...Pippin apples vary in availability, depending on the variety. Most cultivars are harvested in the late fall through early winter and can be stored until the spring. A few Pippin varieties may ripen in mid-summer. Current Facts …The introduction of noninvasive prenatal screening (NIPS) into clinical care in 2011 1, 2 has provided pregnant women around the world with information about their risk for fetal aneuploidies. Whatever the platform used, most NIPS laboratories will report a failure rate between 1% and 5%. 3, 4, 5 This failure is mainly due to a fetal fraction that is too low to make a confident call. 6 At the ...selection with either AMPure PB beads (red) or the BluePippin system (blue). Since the genomic DNA were partially degraded, SMRTbell Libraries were constructed without additional fragmentation. Long continuous read lengths are essential for applications such as de novo assembly and Isoform Sequencing (Iso-Seq).Cassette Kit Product Number Cassette Type Software DNA Control Kit Product Number BLG7510 0.75% and Capture 2 - 5 kb Marker D1 CBC7501 BLG7510 0.75% Band Capture 5 - 10 kb Marker D1 CBC7601

Page 2 P/N 100-286-000-07 STEP Concentrate DNA Notes 1 Add 0.45X volume of AMPure® PB magnetic beads. _____ μL of sample X 0.45X = _____ μL of beads Note that the beads must be brought to room temperature and all AMPure PB bead

Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...

In the set you will find a brightly colored teapot, sugar bowl and milk jug. All products have a blue background and are finished off with pink floral details ...Hi, I am a kind of enthusiast and would like to improve data on my genome with nanopore long reads. Do you possibly know of any lab that performs…The gene structure of Pfpα is shown with exons in blue boxes and introns with a black line. ... and size selection with a Blue Pippin system (Sage Science) to create SMRTbell template libraries ...cDNA-Libraries für Next-Gen Sequencing-Applikationen. Die automatische Größenselektion mit den Pippin Systemen bietet viele Vorteile: High Quality Libraries – ...The desired product was confirmed by running agarose gel (0.8%) electrophoresis and purified by using Blue pippin (Sage Science) (Supplementary Fig. 1b). Single-molecule distance measurement using ...He was an eight-time member of the NBA All-Defensive First Team (1992-1999) and the 1994 All-Star MVP. Yet, his career averages of 16.1 points, 6.4 rebounds and 5.2 assists were not staggering ...Make dark blue paint by mixing blue and black paint together. This takes only a few moments to accomplish. You need blue paint, black paint, white paint, a container and a mixing stick.Sep 19, 2018 · The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10ug (100% up from standard Blue Pippin cassettes). Libraries were size-selected on Blue Pippin (Sage Science, Beverly, MA, USA) instrument using marker S1, with 7 and 10 kb lower cutoff. Size selected DNA was collected and purified using AMPure PB beads (Beckman Coulter, Indianapolis, IN, USA). Sequencing primer was annealed and DNA polymerase bound to the SMRT bell templates. The libraries ...BluePippin Operations Manual 460013 Rev E 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an The bacterial wilt of peanut (Arachis hypogaea L.) caused by Ralstonia solanacearum is a devastating soil-borne disease that seriously restricted the world peanut production.However, the molecular mechanism of R. solanacearum–peanut interaction remains largely unknown.We found that R. solanacearum HA4-1 and PeaFJ1 isolated from peanut …

PULSE1: Pippin Pulse Starter Bundle1, with Gel Box, Notebook, and KBB Buffer: $4,950.00Seal the PCR plate with foil and centrifuge at 1000 x g for 1 minute at room t emper ature. 5. Place the sealed PCR plate into the therm ocycler. 6. Amplify the cpn60 library following the cpn60 ...Buckley lab protocols for conducting SIP studies. Contribute to nick-youngblut/Buckley_Lab_SIP_project_protocols development by creating an account on GitHub.Instagram:https://instagram. jacque vaughwhat is public support servicedoctorate in clinical nutritionku duke basketball Then the large fragments of DNA were recovered by Blue Pippin automatic nucleic acid fragment recovery system, and repaired. Barcode was added by PCR-free method of EXP-NBD104 kit (Oxford Nanopore Technologies Company). The size of fragments was detected by AATI automatic capillary electrophoresis instrument, and the samples were isomolar mixed ... sports coffee table booksla mona nicaragua The authors report on a high quality genome assembly of the model plant species Arabidopsis include two telomere-to-telomere assemblies (Chr 3 and 5). PacBio's HiFi, Oxford Nanopore ultra-long reads and Hi-C (Illumina) were used. BluePippin was used to size select large fragments from genomic extracts and for Hi-FI library construction. cottonwood lawrence ks The BluePippin is an updated version of Sage's Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal ...The Blue Pippin has both standard and pulse field electrophoresis capability permitting a large range of fragment size selection. Cassettes can run up to 5 samples. Unused sample lanes can be used at a later time in some cases. Gel cassettes available are: 3% agarose 100-250bp isolation 2.5% agarose 100-600bp 1.5% agarose 250bp - 1.5Kb